Abstract: Objective To construct a high-fat diet-induced metabolic associated fatty liver disease (MAFLD) model for Myodes rufocanus, so as to provide a new and reliable animal model for the study of MAFLD. Methods Forty male Myodes rufocanus were randomly divided into two groups and fed normal feed ( control group, NCD) and high-fat feed ( high-fat group, HFD) , respectively. Body weight, blood biochemistry, pathological sections and related gene expression levels were detected in each group at week 8 and week 16 after their death. Result In the high-fat group, Myodes rufocanus were gradually obese, depressed, slow-moving, and had a significantly higher body mass, the liver was enlarged in size, blunted at the edges, and yellowed in color, with a significant increase in the hepatic index, ALT, AST, TG, TC, and LDL-C were significantly higher, and HDL-C was significantly lower, liver sections showed steatosis and deposition. In addition, hepatocytes were filled with lipid droplets and infiltrated with inflammatory cells, with a significant increase in NAS score, the expression levels of lipid synthesis genes ( FASN, SREBF1, ACACA) , inflammation-related genes ( IL-6, TNF-α) , and fibrosis-related genes (α-SMA, TGF-β ) were significantly elevated. Conclusion A successful high-fat diet-induced metabolic associated fatty liver disease (MAFLD) model in Myodes rufocanus has been established, which provides a research basis for relevant studies thereafter.

Key words: Myodes rufocanus, metabolic-associated fatty liver disease, high-fat diets, animal model

摘要： 目的 构建高脂饮食诱导的棕背䶄模型, 为代谢相关脂肪性肝病的研究提供新型可靠的动物模型。 方法 取雄性棕背䶄 40 只,随机分为 2 组,分别饲喂普通饲料(对照组)和高脂饲料( 高脂组) ,并于第 8 周和第 16 周安乐死棕背䶄 ,分别检测各组体质量、血生化、病理切片以及相关基因表达水平。 结果 高脂组棕背䶄逐渐肥胖,精神萎靡、行动迟缓,体质量明显升高;肝体积增大、边缘较钝、颜色变黄,肝指数显著增加;ALT、AST、TG、TC、 LDL-C 显著增高,HDL-C 显著降低;肝组织切片可见脂肪变性和沉积,肝细胞充满脂滴且有炎症细胞浸润,NAS 评 分显著增加;脂质合成基因( FASN、SREBF1、ACACA) 、炎症相关基因( IL-6、TNF-α) 以及纤维化相关基因( α-SMA、 TGF-β)表达水平显著升高。 结论 成功构建高脂饮食诱导代谢相关脂肪性肝病棕背䶄模型,为此后相关研究提供依据。

关键词:  , 棕背?, 代谢相关脂肪性肝病, 高脂饮食, 动物模型